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rabbit anti hatag  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti hatag
    Rabbit Anti Hatag, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 4980 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+hatag/HA-Tag+Rabbit+mAb/pm41923141-100-58-62
    Average 99 stars, based on 4980 article reviews
    rabbit anti hatag - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Membrane:

    Article Title: Compartmentalized cAMP Generation by Engineered Photoactivated Adenylyl Cyclases
    Article Snippet: .. Samples were separated on a SuperSep Phos-tag gel (WAKO Chemical cat. #195–17991) following the manufacturer’s instructions and transferred to a PVDF membrane overnight at 4 °C at 20 V. Membranes were dried then rewetted and blocked with Li-Cor TBS Odyssey Blocking Buffer (LI-COR cat. #92750000) for 1 h. Membranes were incubated overnight at 4 °C with 10 mL primary antibodies: 1:1000 Mouse anti-pVasp Ser157 (Abcam ab58555) and 1:2000 Rabbit anti-HAtag (Cell Signaling Technologies cat. #3742) in Odyssey Blocking Buffer + 0.1% Tween-20. .. Membranes were washed three times for 5 min with TBS-Tween and incubated at room temperature for 1 h with 10 mL secondary antibodies: 1:20,000 RDye® 800CW Donkey anti-Mouse IgG (LI-COR cat. #926–32212) and 1:20,000 IRDye® 680RD Donkey anti-Rabbit IgG (LI-COR cat. #926–68973) in Odyssey Blocking Buffer + 0.1% Tween-20 + 0.01% SDS.

    Blocking Assay:

    Article Title: Compartmentalized cAMP Generation by Engineered Photoactivated Adenylyl Cyclases
    Article Snippet: .. Samples were separated on a SuperSep Phos-tag gel (WAKO Chemical cat. #195–17991) following the manufacturer’s instructions and transferred to a PVDF membrane overnight at 4 °C at 20 V. Membranes were dried then rewetted and blocked with Li-Cor TBS Odyssey Blocking Buffer (LI-COR cat. #92750000) for 1 h. Membranes were incubated overnight at 4 °C with 10 mL primary antibodies: 1:1000 Mouse anti-pVasp Ser157 (Abcam ab58555) and 1:2000 Rabbit anti-HAtag (Cell Signaling Technologies cat. #3742) in Odyssey Blocking Buffer + 0.1% Tween-20. .. Membranes were washed three times for 5 min with TBS-Tween and incubated at room temperature for 1 h with 10 mL secondary antibodies: 1:20,000 RDye® 800CW Donkey anti-Mouse IgG (LI-COR cat. #926–32212) and 1:20,000 IRDye® 680RD Donkey anti-Rabbit IgG (LI-COR cat. #926–68973) in Odyssey Blocking Buffer + 0.1% Tween-20 + 0.01% SDS.

    Article Title: Sleep deprivation selectively reactivates hippocampal CA1 pyramidal neurons
    Article Snippet: The brains were then sliced into 30μm sections using a Leica cryostat (Leica CM3050S, IL, USA) at -20°C and stored in cryoprotectant solution (30% w/v sucrose, 30% v/v ethylene glycol, and 0.01% Sodium Azide in 1X PBS). .. Sections containing the dorsal hippocampus were rinsed three times for 5min each in 1X PBS , followed by a 1h incubation in blocking solution (3% normal Donkey/Goat serum in 0.4% triton-X100/PBS buffer) and an overnight incubation at room temperature (RT) with primary antibodies: rabbit anti-c-Fos (1:3000, #226008, Synaptic Systems, Gortingen, Germany), guinea pig anti-c-Fos (1:3000, #226308, Synaptic Systems), rabbit anti-HAtag (1:2000, #3724, Cell Signaling, MA, USA), mouse anti-NeuN (1:1500, #ab104224, Abcam, MA, USA), rabbit anti-Sox9 (1:1500, #ab185966, Abcam). .. Following overnight incubation with primary antibodies, slices were rinsed three times for 5 min in 1X PBS and incubated with secondary antibodies: Alexa Fluor 488 goat anti-rabbit (1:1000, #A11008, Invitrogen, CA, USA), Alexa Fluor 647 donkey anti-rabbit (1:1000, #A32795, Invitrogen), Alexa Fluor 555 goat anti-guinea pig (1:1000, #150186, Abcam), Alexa Fluor 488 donkey anti-mouse (1:1000, #A21202, Invitrogen) for 2h at RT followed by three rinses in 1X PBS for 5min each.

    Article Title: Repeated sleep deprivation selectively reactivates hippocampal CA1 pyramidal neurons.
    Article Snippet: The brains were then sliced into 30μm sections using a Leica cryostat (Leica CM3050S, IL, USA) at -20°C and stored in cryoprotectant solution (30% w/v sucrose, 30% v/v ethylene glycol, and 0.01% Sodium Azide in 1X PBS). .. Sections containing the dorsal hippocampus were rinsed three times for 5min each in 1X PBS , followed by a 1h incubation in blocking solution (3% normal Donkey/Goat serum in 0.4% triton-X100/PBS buffer) and an overnight incubation at room temperature (RT) with primary antibodies: rabbit anti-c-Fos (1:3000, #226008, Synaptic Systems, Goettingen, Germany), guinea pig anti-c-Fos (1:3000, #226308, Synaptic Systems), rabbit anti-HAtag (1:2000, #3724, Cell Signaling, MA, USA), mouse anti-NeuN (1:1500, #ab104224, Abcam, MA, USA), rabbit anti-Sox9 (1:1500, .. AR TIC LE IN PR ES S ARTICLE IN PRESS #ab185966, Abcam), mouse anti-GAD67 (1:1000, #MAB5406, Sigma-Aldrich).

    Incubation:

    Article Title: Compartmentalized cAMP Generation by Engineered Photoactivated Adenylyl Cyclases
    Article Snippet: .. Samples were separated on a SuperSep Phos-tag gel (WAKO Chemical cat. #195–17991) following the manufacturer’s instructions and transferred to a PVDF membrane overnight at 4 °C at 20 V. Membranes were dried then rewetted and blocked with Li-Cor TBS Odyssey Blocking Buffer (LI-COR cat. #92750000) for 1 h. Membranes were incubated overnight at 4 °C with 10 mL primary antibodies: 1:1000 Mouse anti-pVasp Ser157 (Abcam ab58555) and 1:2000 Rabbit anti-HAtag (Cell Signaling Technologies cat. #3742) in Odyssey Blocking Buffer + 0.1% Tween-20. .. Membranes were washed three times for 5 min with TBS-Tween and incubated at room temperature for 1 h with 10 mL secondary antibodies: 1:20,000 RDye® 800CW Donkey anti-Mouse IgG (LI-COR cat. #926–32212) and 1:20,000 IRDye® 680RD Donkey anti-Rabbit IgG (LI-COR cat. #926–68973) in Odyssey Blocking Buffer + 0.1% Tween-20 + 0.01% SDS.

    Article Title: Sleep deprivation selectively reactivates hippocampal CA1 pyramidal neurons
    Article Snippet: The brains were then sliced into 30μm sections using a Leica cryostat (Leica CM3050S, IL, USA) at -20°C and stored in cryoprotectant solution (30% w/v sucrose, 30% v/v ethylene glycol, and 0.01% Sodium Azide in 1X PBS). .. Sections containing the dorsal hippocampus were rinsed three times for 5min each in 1X PBS , followed by a 1h incubation in blocking solution (3% normal Donkey/Goat serum in 0.4% triton-X100/PBS buffer) and an overnight incubation at room temperature (RT) with primary antibodies: rabbit anti-c-Fos (1:3000, #226008, Synaptic Systems, Gortingen, Germany), guinea pig anti-c-Fos (1:3000, #226308, Synaptic Systems), rabbit anti-HAtag (1:2000, #3724, Cell Signaling, MA, USA), mouse anti-NeuN (1:1500, #ab104224, Abcam, MA, USA), rabbit anti-Sox9 (1:1500, #ab185966, Abcam). .. Following overnight incubation with primary antibodies, slices were rinsed three times for 5 min in 1X PBS and incubated with secondary antibodies: Alexa Fluor 488 goat anti-rabbit (1:1000, #A11008, Invitrogen, CA, USA), Alexa Fluor 647 donkey anti-rabbit (1:1000, #A32795, Invitrogen), Alexa Fluor 555 goat anti-guinea pig (1:1000, #150186, Abcam), Alexa Fluor 488 donkey anti-mouse (1:1000, #A21202, Invitrogen) for 2h at RT followed by three rinses in 1X PBS for 5min each.

    Article Title: Repeated sleep deprivation selectively reactivates hippocampal CA1 pyramidal neurons.
    Article Snippet: The brains were then sliced into 30μm sections using a Leica cryostat (Leica CM3050S, IL, USA) at -20°C and stored in cryoprotectant solution (30% w/v sucrose, 30% v/v ethylene glycol, and 0.01% Sodium Azide in 1X PBS). .. Sections containing the dorsal hippocampus were rinsed three times for 5min each in 1X PBS , followed by a 1h incubation in blocking solution (3% normal Donkey/Goat serum in 0.4% triton-X100/PBS buffer) and an overnight incubation at room temperature (RT) with primary antibodies: rabbit anti-c-Fos (1:3000, #226008, Synaptic Systems, Goettingen, Germany), guinea pig anti-c-Fos (1:3000, #226308, Synaptic Systems), rabbit anti-HAtag (1:2000, #3724, Cell Signaling, MA, USA), mouse anti-NeuN (1:1500, #ab104224, Abcam, MA, USA), rabbit anti-Sox9 (1:1500, .. AR TIC LE IN PR ES S ARTICLE IN PRESS #ab185966, Abcam), mouse anti-GAD67 (1:1000, #MAB5406, Sigma-Aldrich).



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